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Thus, no evidence of protein release by the dye was obtained. These results indicate that acceptor activity is sensitive to the intimate structure of chromatin. Modulations in RNA synthesis may then be a consequence of the binding of steroid–receptor complexes to the major groove of the helix or to regulatory proteins located at this surface or both, dianabol steroids price in pakistan. Effect of ethidium bromide on the stability and donor activity of renal cytoplasmic 3 H-aldosterone complexes.
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Dianabol steroids price in pakistan, cheap buy steroids online cycle. Similar findings have been obtained in reconstitution experiments with steroid-labeled cytosol fractions and unlabeled nuclear or chromatin fractions from many tissues [ 1 ], dianabol steroids price in pakistan. One such experiment is shown in Fig. Renal cytoplasmic fractions from adrenalectomized rats were labeled with 3 H-aldosterone and incubated with washed renal nuclei at 25°C. On a quantitative basis nuclear uptake of 3 H-aldosterone complexes (recovered by 01 M Tris–HCl and 0·4 M KCl elution) accounted for 60% of the receptor content lost from the cytoplasmic fraction during incubation [ 19 ]. Moreover, dissociation of the cytoplasmic complex by heating to 37°C eliminated nuclear uptake; in contrast pre-heating the nuclear fraction did not impair uptake.

 

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